Improvement of viral vector purification using Mustang Q membrane chromatography

Saadia Zakai and Mark Schofield of Pall join Hélène Lebas of leading viral vector CDMO, Yposkesi, to discuss key current trends and challenges in AAV and lentiviral vector manufacturing, with a particular focus on the cutting edge in lentiviral vector and adeno-associated viral (AAV) vector purification tools.

Chapters

• 0:57 Introducing the panellists
• 4:37 Key current trends and challenges in AAV and lentiviral vector manufacturing
• 10:27 Characterizing the current viral vector purification toolkits for lentivirus and AAV
• 14:46 What advantages do membrane chromatography and the Mustang Q system offer?
• 17:39 Considerations for Mustang Q application and performance in lentiviral purification
• 21:34 Mustang Q in application for AAV full-empty capsid separation
• 26:32 Will regulators set a minimum specification for the percentage of full AAV capsids?
• 29:05 Parting advice on application of membrane chromatography systems